Whole Genome Dna Amplification Before Applying Wgs

Dear all

We have some DNA samples that I would like to run whole genome sequencing on. Unfortunately these samples only contain very little DNA (too little for WGS). It has then been suggested to do whole genome amplification of the DNA before sequencing. This approach is commonly used before doing genotyping of a specific SNP.

Do you think that the pre-amplification will create a lot of bias in the seqeuncing (i.e generate many false postive genotype call, indell calls, CNV, etc.)?

Another approach could be to do single cell sequencing. What are the pros and cons when applying this?

Thanks a lot for any comments!

best wishes, Thomas